STUDY OF THE EFFECT OF KHAT ON THE BIOAVIALABILITY OF TADALAFIL (EX-VIVO) BY USING EVERTED SAC METHOD

Abstract

Objectives: The study aimed to knowledge and evaluation of  the effect of presence of khat  in the GIT on  the bioavailability and efficacy  of Tadalafil.

Methods: The present study was carried out in ex-vivo by using everted gut sac method, the rabbit was the experimental animal. In comparative studies for absorption of standard tadalafil alone and stand. tadalafil in presence of more than one type of khat (Hishishi and Arhabi) and confirm this study by comparative study for  tadalafil product (Saheal) alone and in presence of the more effective type of khat (Hishishi type). Results: In the proposed studies, by using the calibration curve equation (Regression equation) to calculation of Conc. %,  the means of difference in  Conc.% of stand. tadalafil alone and stand. tadalafil in presence of khat Hishishi and stand. sildenafil in presence of khat Arhabi, were 2.575 ± 0.866 and 1.572 ± 0.0104 respectively,  and the mean of difference in  Conc.% of tadalafil product (Saheal) alone and tadalafil product (Saheal) in the presence of  khat Hishishi was 4.132± 0.008. Conclusions: Based on the results obtained from this study, the tadalafil bioavailability were significantly reduced  in  presence of khat (khat-chewing). The rate of reduction was effected by the type of khat, as al-hishishi khat was more effective than  al-arhabi khat in reduction of bioavailability of tadalafil.