Oil pollution in Lake Timsah, detection and bioremediation through rearing of Mugil cephalus and Tilapia zillii
Abstract
Abstract
This research assessed the capacity of bioremediation the complete pollution of petroleum produced by burned motor oil in the lake Timsah, Egypt using four indigenous bacteria and four isolates of fungi, separated from Lake and to record the effects on two species of fish, Mugil cephalus and Tilapia zillii, Fish to assess the impacts of the water-solution (WSF) fraction of burning motor oil on the growth efficiency and percentage of the fish's survival with regards to their clinical signs, lengths and weights alone and in combination with 4 bacterial and 4 fungal strains, have been carried out on the toxicity and bioremediation research. Burnt motor oil liquids was added to Aquarium water and four bacterial (Achromobacter sp., Bacillus sp., Clostridium sp. and Pseudomonas sp.), four fungal Isolates (Absidia corymbifera, Aspergillus sydowii, Mucor circinelloides, and Penicillium sp.) were taken to treat the water in Lake aquarium with a microbial application. The treatment was prolonged for 45 days. The results showed the great disparity between both types of fish, mullet and tilapia, where the resistance of tilapia over mullet for each of the oil and microorganisms has led the oil added to the aquaria is none treated to the rate of death of 40% of the mullet fish, and 50% of the tilapia processing. A comparison between treatment and blank aquaria the results has shown that the rate of death was more in blank which proves that the micro-organisms have been used in the aquaria of oil additive treatment had improved and grown well. Microbiological analysis was conducted to the muscles and liver of both types of fish and the results showed that the average of CFU of bacterial colonies in fish treated with bacteria is much less compared with untreated fisf. This explain that the growth rate of the tested fish keeping a similar as untreated groups. On the other hand, the GC / FID showed a decrease in the concentration of (PAHs) and this promotes better fish in the treatment groups compared with the control and also do not see any of the compounds of (PAHs) in the aquaria for the treatment of tilapia fish with fungi and bacteria. The results proved that the microbial treatment using combination of fungi and bacteria are more effective for the remediation of the burned motor oil contaminated lake-water than fungi or bacteria alone.
