Antibacterial Susceptibility and Sider Honey Activity against Isolated Bacteria from Wound Patients attending at Al-Gmohori hospital in Hajja City, Yemen

Abstract

Background: The emergence increase of infections caused by resistance bacteria become a real threat to patients and healthcare workers. Aims: The present study was designed to assess the antibacterial susceptibility and Sider honey effect against isolated bacteria from wound infection of patients attending at Al-Gmohori hospital in Hajja City, Yemen. Methods: One hundred and seventy-four swabs specimens were collected from wound patients from July to September 2021. The associated factors were gathered by the intended questionnaire. The identification of bacteria isolates was performed by using standard microbiological methods. Also, the antibacterial activity of some antibiotics and Sider honey were assessed by the disc diffusion method. Results: Out of 176 examined specimens, 124(70.45%) were contaminated by bacteria. The higher rate of bacterial infection was recorded among the age group of 16-25 years (75%), having wound infection in the leg (81.25%), wound period for four weeks (82.35%), from hospital inpatient (82.35%), with abscess (82.61%), injured previously, and not using antibiotics. The most isolated bacteria species from wound infection was Staphylococcus aureus (49.01%) followed by Pseudomonas aeruginosa (23.18%), Escherichia coli (8.61%), Streptococcus pyogenes (4.64%), and Citrobacter sp., and Bacillus sp. (3.97% in each), Klebsiella pneumonia (3.31%), Klebsiella sp. (1.99%), and Streptococcus pneumonia (1.32%). The S. aureus, S. pyogenes, K. pneumonia, and S. pneumonia were sensitive to piperacillin/tazobactam and resistant to cefepime. Also, P. aeruginosa showed high resistance to all used antibiotics. While E. coli was totally sensitive to cefepime and resistant to piperacillin/ tazobactam. Also, Sider honey revealed had highly antimicrobial against P. aeruginosa and S. pyogenes, K. pneumonia bacteria and moderate against S. aureus, E. coli, Bacillus sp. isolates. Conclusion: The obtained results consider the alarming for serious problem for the health system in upcoming years might be resulting from antibiotic-resistant bacteria. Therefore, further experimental are required to evaluate the effectiveness of honey on bacterial resistance.